Sequence-selective osmium oxidation of DNA: efficient distinction between 5-methylcytosine and cytosine.
نویسندگان
چکیده
5-Methylcytosine was distinguished from cytosine using the large difference of their osmium oxidation rates, and this reaction was applied to detection of the cytosine methylation status at a specific site of a long sequence using the formation of a bulge structure by hybridization with a guide DNA.
منابع مشابه
Facile synthesis of hydroxymethylcytosine-containing oligonucleotides and their reactivity upon osmium oxidation.
DNA strands containing a 5-hydroxymethylcytosine ((hm)C), which have recently been found in neuron cells and embryonic stem cells, were synthesized through a facile synthetic technique. The (hm)C-containing strands were efficiently oxidized at (hm)C using an osmium oxidation assay. The (hm)C was oxidized as easily as 5-methylcytosine, which can be distinguished from unmethylated cytosine.
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عنوان ژورنال:
- Organic & biomolecular chemistry
دوره 4 9 شماره
صفحات -
تاریخ انتشار 2006